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How To Sanitize, Collect and Submit Samples

*** PLEASE USE A 100 ml PLASTIC STERILE BOTTLE FROM OUR LAB ***

* SELECTING A TAP:

  • A Holding tank faucet is preferred. Petcock or small valve is acceptable.
  • DO NOT sample from hoses or drinking fountains.
  • Avoid taps with leaks at faucet or stem.
  • Avoid taps with SWIVEL JOINTS.
  • The nearest tap to the well is preferred if you want to check the well for bacteria.
  • Bathroom sink is better than a swivel kitchen sink.

    * STERILIZE THE FAUCET:

  • Remove aeration screen from inside of cold water faucet.
  • Take a quart plastic bag - put 2 cups of water and 1 ounce of bleach in bag. Attach the bag to open end of the faucet. Use your hand to clamp and seal around the faucet. Carefully lift up and squeeze the bottom of the suspended bag 3 X to sanitize the opening and inside of the faucet.
  • Remove the bag and empty into drain - DO NOT touch the faucet opening.

    * FLUSH THE TAP:

  • Turn on Cold Water fully open as possible.
  • Let Run 3 Minutes minimum.

    * TAKING THE SAMPLE:

  • Reduce water flow to pencil width.
  • Remove the sealed cap from bottle. DO NOT touch inside of lid or mouth of bottle.
  • Do not rinse bottle out.
  • Fill bottle to the line marked 100 ml. Do not over fill bottle.
  • Immediatly recap bottle tightly, and keep the sample cool.

    * SUBMITTING SAMPLES:

  • Label your bottle with a sample description.
  • Complete the Yellow Information Form.
  • Return the water sample(s) along with the Sample Information Form, and Payment to: HOLMES LABORATORY, INC. - P.O. BOX 204 - WINESBURG, OH 44690